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SRpHi2
(Plasmid #102542)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102542 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tat-EGFP
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (not destroyed)
  • 3′ cloning site Unknown (not destroyed)
  • 5′ sequencing primer pGEX5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

TAT-peptide is N-terminal to, and in frame with EGFP (between GST and EGFP); BL-21 cells are used for protein expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SRpHi2 was a gift from Stefan Hell (Addgene plasmid # 102542 ; http://n2t.net/addgene:102542 ; RRID:Addgene_102542)
  • For your References section:

    SRpHi ratiometric pH biosensors for super-resolution microscopy. Richardson DS, Gregor C, Winter FR, Urban NT, Sahl SJ, Willig KI, Hell SW. Nat Commun. 2017 Sep 18;8(1):577. doi: 10.1038/s41467-017-00606-4. 10.1038/s41467-017-00606-4 [pii] PubMed 28924139