Purposebacterial expression of AsCpf1-2NLS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102565||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 10750
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesSynthetic; Acidaminococcus sp.
Insert Size (bp)5230
- Promoter T7
/ Fusion Protein
- MBP (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Pro (Common Sequencing Primers)
"CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing" - Moreno-Mateos et al.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AsCpf1-2NLS was a gift from Jennifer Doudna (Addgene plasmid # 102565 ; http://n2t.net/addgene:102565 ; RRID:Addgene_102565)
For your References section:CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2. 10.1038/s41467-017-01836-2 [pii] PubMed 29222508