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(Plasmid #102678)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102678 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 7166
  • Total vector size (bp) 9211
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    CHANGE Glutamate 324 to Alanine
  • GenBank ID
  • Entrez Gene
    RIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
  • Promoter unknown
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Mfe I/Ecor I (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The RIPK1 insert has a K87Q polymorphism which has no effect on protein activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-Flag-RIPK1-D324A was a gift from Hasam Habelhah (Addgene plasmid # 102678 ; ; RRID:Addgene_102678)
  • For your References section:

    RIP1 Cleavage in the Kinase Domain Regulates TRAIL-Induced NF-kappaB Activation and Lymphoma Survival. Zhang L, Blackwell K, Workman LM, Chen S, Pope MR, Janz S, Habelhah H. Mol Cell Biol. 2015 Oct;35(19):3324-38. doi: 10.1128/MCB.00692-15. Epub 2015 Jul 20. 10.1128/MCB.00692-15 PubMed 26195820