pmTurqoise KIF1A shRNA
PurposeGenerates shRNA specific to Rat KIF1A along with cell fill pmTurqoise
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102849||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5415
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameATSV, Kns1
SpeciesR. norvegicus (rat)
- Promoter H1-RNA
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer T7 TAATACGACTCACTATAGGG , M13R CAGGAAACAGCTATGAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The pmTurquoise KIF1A shRNA vector was constructed by restriction site removal of eGFP from the commercially available pSuper vector (Oligoengine) and insertion of the cDNA coding for pmTurquoise (Addgene plasmid #36202).
The reference for the pSuper Vector (Oligoengine) is T.R. Brummelkamp, R. Bernards, and R Agami, Science 296, 550 (2002).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmTurqoise KIF1A shRNA was a gift from Erik Dent (Addgene plasmid # 102849 ; http://n2t.net/addgene:102849 ; RRID:Addgene_102849)
For your References section:Transport of a kinesin-cargo pair along microtubules into dendritic spines undergoing synaptic plasticity. McVicker DP, Awe AM, Richters KE, Wilson RL, Cowdrey DA, Hu X, Chapman ER, Dent EW. Nat Commun. 2016 Sep 23;7:12741. doi: 10.1038/ncomms12741. 10.1038/ncomms12741 PubMed 27658622