tdTomato-KIF1A
(Plasmid #102850)

• Purpose: Overexpresses KIF1A and cell fill TdTomato
• Depositing Lab: Erik Dent
• Publication: McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10.1038/ncomms12741.

Backbone

• Vector backbone: peGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 9902
• Modifications to backbone: EGFP replaced by tdTomato
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF1A
• Alt name: ATSV, Kns1
• Species: R. norvegicus (rat)
• Insert Size (bp): 5085
• Entrez Gene: Kif1a (a.k.a. A, ATSV, C630002N23Rik, Gm1626, Kn, Kns1)
• Promoter: CMV
• Tag / Fusion Protein:
  • tdTomato (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI or AgeI (unknown if destroyed)
• 3′ cloning site: ScaI (unknown if destroyed)
• 5′ sequencing primer: tdTomato-Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The rat eGFP-KIF1A was a kind gift from Gary Banker (OHSU).

The tdTomato-KIF1A plasmid was constructed by restriction site removal of the eGFP from the eGFP-KIF1A plasmid and insertion of tdTomato.

Addgene NGS results found S33N within the KIF1A translation compared to NCBI reference sequence XP_017459420.1