PurposeExpress DNA binding domain of mouse CTCF in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102859||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneNEB DHFR control plasmid
- Total vector size (bp) 3447
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)849
Entrez GeneCtcf (a.k.a. AW108038)
- Promoter T7
/ Fusion Protein
- hisSUMO (N terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
This plasmid encodes the DNA binding domain of mouse CTCF protein (F1 to F9) for E. coli expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hisSUMO-CTCF(F1-F9) was a gift from Gary Stormo (Addgene plasmid # 102859 ; http://n2t.net/addgene:102859 ; RRID:Addgene_102859)
For your References section:Measuring quantitative effects of methylation on transcription factor-DNA binding affinity. Zuo Z, Roy B, Chang YK, Granas D, Stormo GD. Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. eCollection 2017 Nov. aao1799 [pii] PubMed 29159284