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hisSUMO-CTCF(F1-F9)
(Plasmid #102859)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102859 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    NEB DHFR control plasmid
  • Total vector size (bp) 3447
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CTCF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    849
  • Entrez Gene
    Ctcf (a.k.a. AW108038)
  • Promoter T7
  • Tag / Fusion Protein
    • hisSUMO (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

This plasmid encodes the DNA binding domain of mouse CTCF protein (F1 to F9) for E. coli expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hisSUMO-CTCF(F1-F9) was a gift from Gary Stormo (Addgene plasmid # 102859 ; http://n2t.net/addgene:102859 ; RRID:Addgene_102859)
  • For your References section:

    Measuring quantitative effects of methylation on transcription factor-DNA binding affinity. Zuo Z, Roy B, Chang YK, Granas D, Stormo GD. Sci Adv. 2017 Nov 17;3(11):eaao1799. doi: 10.1126/sciadv.aao1799. eCollection 2017 Nov. aao1799 [pii] PubMed 29159284