pCS2+ mWnt5a
(Plasmid #102860)

• Purpose: Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus
• Depositing Lab: Henry Ho
• Publication: Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4085
• Total vector size (bp): 5227
• Modifications to backbone: Addition of FseI and AscI sites
• Vector type: Mammalian Expression ; zebrafish expression; Xenopus expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mouse Wnt5a
• GenBank ID: NP_033550.2
• Promoter: CMV; SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: SP6 (ATTTAGGTGACACTATA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry