pZL1-ZF - myelin basic protein a (mbpa)
(Plasmid #103007)

• Purpose: Plasmid template for making in-situ hybridization probe for myelin basic protein a (mbpa) gene zf-fj330a07.y1.
• Depositing Lab: Marnie Halpern
• Publication: Brosamle et al Glia. 2002 Jul;39(1):47-57.

Backbone

• Vector backbone: pZL1
• Backbone size w/o insert (bp): 4308

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The lab grows in 50ug/ml carbenicllin using DH10beta cells.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myelin basic protein
• Alt name: mbp probe
• Species: D. rerio (zebrafish)
• GenBank ID: AW077205.1
• Entrez Gene: mbpa (a.k.a. cb274, fj33b11, mbp, wu:fj33b11, wu:fq15b02, zgc:136630)
• Promoter: Sp6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (unknown if destroyed)
• 3′ cloning site: Sal1 (unknown if destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RZPD Berlin

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry