pZL1-ZF - myelin basic protein a (mbpa)
PurposePlasmid template for making in-situ hybridization probe for myelin basic protein a (mbpa) gene zf-fj330a07.y1.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||103007||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4308
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsThe lab grows in 50ug/ml carbenicllin using DH10beta cells.
Copy numberHigh Copy
Gene/Insert namemyelin basic protein
Alt namembp probe
SpeciesD. rerio (zebrafish)
Entrez Genembpa (a.k.a. cb274, fj33b11, mbp, wu:fj33b11, wu:fq15b02, zgc:136630)
- Promoter Sp6
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (unknown if destroyed)
- 3′ cloning site Sal1 (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRZPD Berlin
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZL1-ZF - myelin basic protein a (mbpa) was a gift from Marnie Halpern (Addgene plasmid # 103007 ; http://n2t.net/addgene:103007 ; RRID:Addgene_103007)
For your References section:Characterization of myelination in the developing zebrafish. Brosamle C, Halpern ME. Glia. 2002 Jul;39(1):47-57. 10.1002/glia.10088 PubMed 12112375