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pLenti CMV-MRLC1-mRuby2-IRES-PuroR
(Plasmid #103031)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 103031 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCIG3 #78264
  • Backbone manufacturer
    Goodrum Lab, University of Arizona
  • Backbone size w/o insert (bp) 8161
  • Total vector size (bp) 8677
  • Modifications to backbone
    Subcloned in Puromycin resistance gene in place of GFP behind the IRES sequence
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MRLC1
  • Alt name
    MYL9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    516
  • GenBank ID
    NM_006097.4
  • Entrez Gene
    MYL9 (a.k.a. LC20, MLC-2C, MLC2, MRLC1, MYRL2)
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tom Egelhoff, Addgene #35680
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV-MRLC1-mRuby2-IRES-PuroR was a gift from Ghassan Mouneimne (Addgene plasmid # 103031 ; http://n2t.net/addgene:103031 ; RRID:Addgene_103031)
  • For your References section:

    The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, Knudsen B, Mouneimne G. Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2. 10.1038/s41467-018-05367-2 [pii] PubMed 30061623