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pCAGGS_3E5E_luciferase
(Plasmid #103055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 103055 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGGs
  • Total vector size (bp) 7785
  • Modifications to backbone
    firefly luciferase reporter gene under control of the CAG promoter
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ebolavirus minigenome
  • Species
    Zaire ebolavirus, Mayinga variant
  • Promoter CAG

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS_3E5E_luciferase was a gift from Elke Mühlberger (Addgene plasmid # 103055 ; http://n2t.net/addgene:103055 ; RRID:Addgene_103055)
  • For your References section:

    An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening. Nelson EV, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Ruedas JB, Connor JH, Ebihara H, Muhlberger E. Antiviral Res. 2017 Aug 12;146:21-27. doi: 10.1016/j.antiviral.2017.08.005. 10.1016/j.antiviral.2017.08.005 PubMed 28807685