PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||103051||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
- Total vector size (bp) 6563
Modifications to backbonepCAGGS containing additonal multiple cloning sites
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameVP30 gene of Ebola virus
SpeciesZaire ebolavirus, Mayinga
Entrez GeneVP30 (a.k.a. ZEBOVgp5)
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 3′ cloning site Xho 1 (unknown if destroyed)
- 5′ sequencing primer CAG-F GCAACGTGCTGGTTATTGTG (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS_VP30_EBOV was a gift from Elke Mühlberger (Addgene plasmid # 103051 ; http://n2t.net/addgene:103051 ; RRID:Addgene_103051)
For your References section:An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening. Nelson EV, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Ruedas JB, Connor JH, Ebihara H, Muhlberger E. Antiviral Res. 2017 Aug 12;146:21-27. doi: 10.1016/j.antiviral.2017.08.005. 10.1016/j.antiviral.2017.08.005 PubMed 28807685