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b2-TS5
(Plasmid #103878)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103878 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 103878-D50 50 μg of DNA in Tris buffer $495
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DNA 103878-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1/hygro(-)
  • Backbone size w/o insert (bp) 5400
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    full-length human b2 with tension sensor at b2_743
  • Alt name
    b2-TS5
  • Alt name
    beta2-TS5
  • Alt name
    integrin subunit beta 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Entrez Gene
    ITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
  • Tag / Fusion Protein
    • tension sensor module (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: this plasmid is identical to pcDNA3.1/Hygro(-)-B2-TS5 (Plasmid #104354).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    b2-TS5 was a gift from Timothy Springer (Addgene plasmid # 103878 ; http://n2t.net/addgene:103878 ; RRID:Addgene_103878)

  • For your References section:

    Coordinated integrin activation by actin-dependent force during T-cell migration. Nordenfelt P, Elliott HL, Springer TA. Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119. 10.1038/ncomms13119 PubMed 27721490

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