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(Plasmid #104070)


Item Catalog # Description Quantity Price (USD)
Plasmid 104070 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Liu Lab
  • Total vector size (bp) 3838
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Plpp MbAcK3RS(IPYE); PproK pyl
  • Species
  • Mutation
    Contains activating mutations V31I, T56P, H62Y, A100E (IPYE) in the MbAcK3RS gene
  • Promoter Plpp and PproK

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTECH-MbAcK3RS(IPYE) was a gift from David Liu (Addgene plasmid # 104070 ; ; RRID:Addgene_104070)
  • For your References section:

    Continuous directed evolution of aminoacyl-tRNA synthetases. Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR. Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. 10.1038/nchembio.2474 PubMed 29035361