pBAF
(Plasmid #104152)

• Purpose: Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.
• Depositing Lab: Robert Craigie
• Publication: Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1528-33.

Backbone

• Vector backbone: pET15B
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Propagate plasmid in strain such as DH5alpha. Use BL21 for expression. Do not allow cultures to reach stationary phase as this may result in loss of plasmid
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Barrier-to-autointegration factor (BAF)
• Alt name: BANF1
• Species: H. sapiens (human)
• Insert Size (bp): 267
• GenBank ID: AF070447.1
• Entrez Gene: BANF1 (a.k.a. BAF, BCRP1, D14S1460, NGPS)
• Tag / Fusion Protein:
  • N-terminal His tag with thrombin cleavage site (N terminal on insert)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry