-
PurposeBacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepQE80 derivative
- Total vector size (bp) 4647
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAnti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
-
SpeciesVicugna pacos
-
Mutation1x Cysteine located at bps 493-495 in full sequence.
-
Tags
/ Fusion Proteins
- 14x Histidine tag (N terminal on backbone)
- NEDD8 from Brachypodium distachyon (cleavable by NEDP1) (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTP261 was a gift from Dirk Görlich (Addgene plasmid # 104166 ; http://n2t.net/addgene:104166 ; RRID:Addgene_104166) -
For your References section:
Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. Pleiner T, Bates M, Trakhanov S, Lee CT, Schliep JE, Chug H, Bohning M, Stark H, Urlaub H, Gorlich D. Elife. 2015 Dec 3;4:e11349. doi: 10.7554/eLife.11349. 10.7554/eLife.11349 PubMed 26633879
