PurposeExpresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GST
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104194||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 5900
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL-1 blue
Copy numberHigh Copy
Gene/Insert namehuman androgen receptor aa 1-333
SpeciesH. sapiens (human)
Insert Size (bp)1000
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer don't have
- 3′ sequencing primer don't have (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The GST-AR insert contains an additional 4 glutamine residues at amino acid 74 compared to AAA51886.1 and the depositor has confirmed that this does not impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-AR-1-333 was a gift from Elizabeth Wilson (Addgene plasmid # 104194 ; http://n2t.net/addgene:104194 ; RRID:Addgene_104194)
For your References section:FXXLF and WXXLF sequences mediate the NH2-terminal interaction with the ligand binding domain of the androgen receptor. He B, Kemppainen JA, Wilson EM. J Biol Chem. 2000 Jul 28;275(30):22986-94. 10.1074/jbc.M002807200 PubMed 10816582