PurposeExpresses ompR from a TetR reuglated promoter under inducible ATC control.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104335||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3624
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesEscherichia coli str. K-12 substr. MG1655
Insert Size (bp)720
Entrez GeneompR (a.k.a. b3405, ECK3392, cry, kmt, ompB)
- Promoter PLTetO1
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer TTGGAACCTCTTACGTGCCG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSR26-2 was a gift from Jeffrey Tabor (Addgene plasmid # 104335 ; http://n2t.net/addgene:104335 ; RRID:Addgene_104335)
For your References section:Phosphatase activity tunes two-component system sensor detection threshold. Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ. Nat Commun. 2018 Apr 12;9(1):1433. doi: 10.1038/s41467-018-03929-y. 10.1038/s41467-018-03929-y PubMed 29650958