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(Plasmid #104406)


Item Catalog # Description Quantity Price (USD)
Plasmid 104406 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Keith Joung (Addgene plasmid # 47754)
  • Backbone size w/o insert (bp) 9075
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    IRATp970A0364D (Source Bioscience) has a P405H change that was reverted to the consensus
  • Promoter CMV promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). LSD1A isoform B catalytic domain (a.a. 171-852) was from LSD1 cDNA clone IRATp970A0364D (Source Bioscience). The P405H change in this cDNA clone was reverted to the consensus during Gibson assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-dCas9-LSD1-Hygro was a gift from Stephan Beck (Addgene plasmid # 104406 ; ; RRID:Addgene_104406)