|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104414||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKeith Joung (Addgene plasmid # 47754)
- Backbone size w/o insert (bp) 9075
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationIRATp970A0364D (Source Bioscience) has a P405H change that was reverted to the consensus. K661A
- Promoter CMV promoter
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). LSD1A isoform B catalytic domain (a.a. 171-852) was from LSD1 cDNA clone IRATp970A0364D (Source Bioscience). The P405H change in this cDNA clone was reverted to the consensus during Gibson assembly. K661A mutation was introduced by PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p-dCas9-LSD1-K661A-Hygro was a gift from Stephan Beck (Addgene plasmid # 104414 ; http://n2t.net/addgene:104414 ; RRID:Addgene_104414)