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pRW004-SM-destination-proUBQ10-Cas9
(Plasmid #104438)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104438 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZ001
  • Backbone manufacturer
    Lampropoulos et al
  • Backbone size (bp) 4114
  • Modifications to backbone
    Cassettes of pro::pcoCas9 and At2S3::mcherry are engineered into the backbone; and the cassette of ccdB-Chloramphenicol is replaced with proLacZ::LacZ expressing unit
  • Vector type
    Bacterial Expression, Plant Expression, CRISPR
  • Promoter UBQ10
  • Selectable markers
    red fluorescence

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRW004-SM-destination-proUBQ10-Cas9 was a gift from Detlef Weigel (Addgene plasmid # 104438 ; http://n2t.net/addgene:104438 ; RRID:Addgene_104438)
  • For your References section:

    An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, Schwab R, Weigel D. Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. eCollection 2018. 10.1186/s13007-018-0330-7 PubMed 30083222