Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #104442)


Item Catalog # Description Quantity Price (USD)
Plasmid 104442 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4754
  • Total vector size (bp) 7832
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    N43A in GST (in a potential KFERQ-like sequence). BHMT does not contain the first 8 or last 9 amino acids.
  • Entrez Gene
    BHMT (a.k.a. BHMT1, HEL-S-61p)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • GFP-myc GFP is from Aequorea victoria (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nru1 (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer SP6 5'-TGATGAGTTTGGACAAACCAC-3'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP is from Aequorea victoria

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-BHMT-I/G was a gift from Carol Mercer (Addgene plasmid # 104442 ; ; RRID:Addgene_104442)
  • For your References section:

    Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization. Mercer CA, Kaliappan A, Dennis PB. Autophagy. 2008 Feb;4(2):185-94. Epub 2007 Nov 7. 5275 [pii] PubMed 18059170