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pACYCduet-mxLOX1-EH
(Plasmid #104978)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104978 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYCDuet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4008
  • Total vector size (bp) 7034
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    MXAN_1745
  • Alt name
    mxLOX1
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    2148
  • GenBank ID
    ABF 86480.1
  • Entrez Gene
    MXAN_1745 (a.k.a. MXAN_1745)
  • Promoter T7promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GATCGAATTCATGAGCGCGAGTGTGACCCGGA
  • 3′ sequencing primer GCCGCAAGCTTTTAGATATTGATGCGGGAACTGA
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    MXAN_1644
  • Alt name
    mxEH
  • Species
    Myxococcus xanthus
  • Insert Size (bp)
    957
  • GenBank ID
    ABF 91519.1
  • Entrez Gene
    MXAN_1644 (a.k.a. MXAN_1644)
  • Promoter T7promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GCGCATATGGCTGACATCACGCATCGAACCGT
  • 3′ sequencing primer GCGCTCGAGTCAGGCCGGCAGCTTCTTCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYCduet-mxLOX1-EH was a gift from Deokkun Oh (Addgene plasmid # 104978 ; http://n2t.net/addgene:104978 ; RRID:Addgene_104978)