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Addgene

pFA6a-3YFP-kanMX6
(Plasmid #105149)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105149 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Backbone size w/o insert (bp) 4170
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    KanMX6 (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3YFP-kanMX6
  • Alt name
    triple YFP
  • Insert Size (bp)
    2160
  • Tag / Fusion Protein
    • 3YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sizes of backbone and inserts are approximate, and do not include the selectable marker.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-3YFP-kanMX6 was a gift from David Kovar & Thomas Pollard & Jian-Qiu Wu (Addgene plasmid # 105149 ; http://n2t.net/addgene:105149 ; RRID:Addgene_105149)
  • For your References section:

    Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. Wu JQ, Sirotkin V, Kovar DR, Lord M, Beltzner CC, Kuhn JR, Pollard TD. J Cell Biol. 2006 Jul 31;174(3):391-402. doi: 10.1083/jcb.200602032. Epub 2006 Jul 24. 10.1083/jcb.200602032 PubMed 16864655