Trpc2-IRES-taumCherry-ACNF TV
(Plasmid #105212)

• Purpose: targeting vector to generate a Trpc2-IRES-taumCherry mouse strain.
• Depositing Lab: Peter Mombaerts
• Publication: Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.

Backbone

• Vector backbone: pBluescript-II-KS
• Backbone size w/o insert (bp): 3000
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trpc2-IRES-taumCherry-ACNF
• Alt name: Trpc2
• Species: M. musculus (mouse)
• Insert Size (bp): 15894
• Entrez Gene: Trpc2 (a.k.a. 3010009O07Rik, AI115608, TRPC2a, TRPC2b, Trrp2, mTrp2, smTRPC2, trp2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13-20
• 3′ sequencing primer: M13-Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Linearize the TV with SalI for electroporation.