Ptrc::RpaA D53A
(Plasmid
#105229)
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Purposeinducible expression of unphosphorylatable variant of RpaA (D53A)
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Depositing Lab
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Depositing OrganizationHarvard University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 105229-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 105229-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneNS2.2-targetting vector (into neutral site NS2.2)
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 10000
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Vector typeCyanobacteria cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerpaA driven by Ptrc promoter
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SpeciesNA
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Insert Size (bp)4000
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MutationD53 mutated to A
- Promoter ptrc
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bygift from a lab member Joseph Markson
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 105229-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 105229-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ptrc::RpaA D53A was a gift from Erin O'Shea (Addgene plasmid # 105229 ; http://n2t.net/addgene:105229 ; RRID:Addgene_105229) -
For your References section:
Switching of metabolic programs in response to light availability is an essential function of the cyanobacterial circadian output pathway. Puszynska AM, O'Shea EK. Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210. 10.7554/eLife.23210 PubMed 28430105
