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PurposeKnock in H2B-GFP into the human PAX6 locus
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 105239-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 105239-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 7158
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Vector typeTALEN
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
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SpeciesH. sapiens (human)
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Insert Size (bp)4463
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byH2B-GFP was a gift from Geoff Wahl (Addgene plasmid # 11680)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 105239-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 105239-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAX6 donor plasmid was a gift from Lorenz Studer (Addgene plasmid # 105239 ; http://n2t.net/addgene:105239 ; RRID:Addgene_105239) -
For your References section:
A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages. Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, Studer L. Cell Stem Cell. 2017 Sep 7;21(3):399-410.e7. doi: 10.1016/j.stem.2017.08.015. 10.1016/j.stem.2017.08.015 PubMed 28886367
Map uploaded by the depositor.
