pEGFP Tensin1
(Plasmid #105297)

• Purpose: To study localization and function of Tensin1
• Depositing Labs: David Critchley, Kenneth Yamada
• Publication: Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tensin1
• Alt name: TNS1
• Alt name: NM_001308023
• Species: H. sapiens (human)
• Insert Size (bp): 5200
• Entrez Gene: TNS1 (a.k.a. MST091, MST122, MST127, MSTP091, MSTP122, MSTP127, MXRA6, PPP1R155, TNS)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: Sal1 (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: This plasmid has several mutations compared to reference sequence NM_001308023, including the deletion of amino acid 8, R143Q, W1176R, and V1583I. The depositor has confirmed that these do not affect plasmid function.