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Addgene

pmCherry Tensin3
(Plasmid #105301)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105301 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmCherry NBC1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tensin3
  • Alt name
    TNS3
  • Alt name
    NM_022748
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4300
  • Entrez Gene
    TNS3 (a.k.a. TEM6, TENS1)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human tensin 1 was from Dr. Katherine Clark of University of Leicester, UK

Note: The TNS3 insert contains a C1276Y mutation compared to the listed reference sequence. The depositor has confirmed that this does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry Tensin3 was a gift from Kenneth Yamada (Addgene plasmid # 105301 ; http://n2t.net/addgene:105301 ; RRID:Addgene_105301)
  • For your References section:

    Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1. Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, Critchley DR. J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460. 10.1002/jcb.22460 PubMed 20069572