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pmCherry Tensin3
(Plasmid #105301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105301 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 105301-D50 50 μg of DNA in Tris buffer $495
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DNA 105301-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmCherry NBC1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tensin3
  • Alt name
    TNS3
  • Alt name
    NM_022748
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4300
  • Entrez Gene
    TNS3 (a.k.a. TEM6, TENS1)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human tensin 1 was from Dr. Katherine Clark of University of Leicester, UK

Note: The TNS3 insert contains a C1276Y mutation compared to the listed reference sequence. The depositor has confirmed that this does not affect plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry Tensin3 was a gift from Kenneth Yamada (Addgene plasmid # 105301 ; http://n2t.net/addgene:105301 ; RRID:Addgene_105301)

  • For your References section:

    Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1. Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, Critchley DR. J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460. 10.1002/jcb.22460 PubMed 20069572
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