pCMV iGluu
(Plasmid #105832)

• Purpose: Fast indicator for extracellular glutamate imaging
• Depositing Lab: Katalin Torok
• Depositing Organization: City, University of London
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115.

Backbone

• Vector backbone: pCMV(MinDis) [variant of pDisplay]
• Backbone manufacturer: modified from Invitrogen
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6829
• Modifications to backbone: From pDisplay: Hemagglutinin (HA) tag removed
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iGluSnFR S72T variant
• Alt name: iGluu
• Species: Escherichia coli (strain K12)
• Insert Size (bp): 1570
• Mutation: Changed Ser 72 to Thr
• GenBank ID: 21239023 21239023
• Promoter: CMV
• Tags / Fusion Proteins:
  • PDGFR (C terminal on insert)
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCMV(MinDis).iGluSnFR vector was obtained from Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCMV iGluu is the S72T variant of iGluSnFR.
(iGluSnFR was created by Marvin et al.2013)

bioRxiv: https://doi.org/10.1101/233494

DNA (Catalog # 105832-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 105832-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.