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pUCM-CLYBL-hNIL
(Plasmid #105841)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105841 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUCM
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 13284
  • Vector type
    CRISPR, TALEN
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Neurogenin 2
  • Alt name
    NGN2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    819
  • GenBank ID
    NM_024019
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Promoter TRE3G

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ISL LIM Homeobox 1
  • Alt name
    ISL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1050
  • GenBank ID
    NM_002202
  • Promoter TRE3G

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    LIM Homeobox 3
  • Alt name
    LHX3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1209
  • Promoter TRE3G

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGCATGGTAGCCAGTCCTATTG
  • 3′ sequencing primer TGTGGAATTGTGAGCGGATA
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    mCherry
  • Alt name
    mcherry
  • Species
    Synthetic
  • Insert Size (bp)
    702
  • Promoter EF-1alpha

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    rtTA3G
  • Alt name
    rtTA3G
  • Insert Size (bp)
    747
  • Promoter CAG

Cloning Information for Gene/Insert 5

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUCM-CLYBL-hNIL was a gift from Michael Ward (Addgene plasmid # 105841 ; http://n2t.net/addgene:105841 ; RRID:Addgene_105841)
  • For your References section:

    Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons. Fernandopulle MS, Prestil R, Grunseich C, Wang C, Gan L, Ward ME. Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18. 10.1002/cpcb.51 PubMed 29924488