AXL KD
(Plasmid
#105934)
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PurposeMammalian expression of myc-his tagged AXL kinase dead (K558R) mutant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA4/TO/myc/HIS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAXL
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SpeciesH. sapiens (human)
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MutationK558R, kinase dead
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GenBank IDAAH32229.1
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Entrez GeneAXL (a.k.a. ARK, AXL3, JTK11, Tyro7, UFO)
- Promoter tet-inducible CMV
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Tags
/ Fusion Proteins
- myc (C terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AXL KD was a gift from Rosa Marina Melillo (Addgene plasmid # 105934 ; http://n2t.net/addgene:105934 ; RRID:Addgene_105934) -
For your References section:
Activation of TYRO3/AXL tyrosine kinase receptors in thyroid cancer. Avilla E, Guarino V, Visciano C, Liotti F, Svelto M, Krishnamoorthy G, Franco R, Melillo RM. Cancer Res. 2011 Mar 1;71(5):1792-804. doi: 10.1158/0008-5472.CAN-10-2186. Epub 2011 Feb 22. 10.1158/0008-5472.CAN-10-2186 PubMed 21343401
