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pME mKate2-dynactin1-811
(Plasmid #105971)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105971 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dynactin1-811
  • Species
    H. sapiens (human)
  • Mutation
    contains only amino acids 1-811
  • Entrez Gene
    DCTN1 (a.k.a. DAP-150, DP-150, P135)
  • Tag / Fusion Protein
    • mKate2 (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Depositor Comments

The depositor notes that the mutations found in dynactin are not of functional concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME mKate2-dynactin1-811 was a gift from Caren Norden (Addgene plasmid # 105971 ; http://n2t.net/addgene:105971 ; RRID:Addgene_105971)
  • For your References section:

    Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB. Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206. 10.1038/srep21206 PubMed 26879757