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Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells.
Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB
Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
105969hsp70:Stathmin1-mKate2 heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326
105970hsp70: mKate2 dynactin1-811heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
105971pME mKate2-dynactin1-811entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811
105972hsp70: mKate2-DN Kif5aheat shock inducible transgenesis
105973p3E DN Kif5a 3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp