hsp70: mKate2 dynactin1-811
(Plasmid #105970)

• Purpose: heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
• Depositing Lab: Caren Norden
• Publication: Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.

Backbone

• Vector backbone: GWpTolDest(R4-R2)pA
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dynactin1-811
• Species: H. sapiens (human)
• Mutation: contains only amino acids 1-811
• Entrez Gene: DCTN1 (a.k.a. DAP-150, DP-150, P135)
• Tag / Fusion Protein:
  • mKate2 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • 320_Tol2_hsp70-mKate2-dynactin1-811.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor notes that the L287M found in dynactin is not of functional concern.