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pFRT-TO-hNANOS3opt-VH
(Plasmid #106341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106341 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFRT-TO-DEST
  • Vector type
    Mammalian Expression ; Gateway entry vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NANOS3
  • Alt name
    NANOS3-VH
  • Alt name
    NANOS3-V5-HA
  • Species
    H. sapiens (human)
  • Mutation
    codon optimized for gene synthesis without affecting amino acid sequence
  • Entrez Gene
    NANOS3 (a.k.a. NANOS1L, NOS3, ZC2HC12C)
  • Tag / Fusion Protein
    • V5, HA (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-TO-hNANOS3opt-VH was a gift from Thomas Tuschl (Addgene plasmid # 106341 ; http://n2t.net/addgene:106341 ; RRID:Addgene_106341)
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