Thomas Tuschl Lab: Thomas Tuschl Lab Plasmids 2018
Tuschl Lab
unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106335 | pENTR4-hDND1_opt | Gateway Entry vector for DND1 (codon optimized for efficient gene synthesis) |
| 106336 | pENTR4-hNANOS3_opt | Gateway Entry vector for NANOS3 (codon optimized for efficient gene synthesis) |
| 106337 | pENTR4-hNANOS3opt-VH | Gateway Entry vector for NANOS3 C-terminally tagged with V5-HA (codon optimized for efficient gene synthesis). |
| 106338 | pENTR4-hNANOS3opt-VH-FH-DND1opt | Gateway Entry vector for NANOS3 C-terminally tagged with V5-HA (codon optimized for efficient gene synthesis) in Flp-In T-REx system. |
| 106339 | pFRT-TO_FH_EGFP_opt | Mammalian expression vector for FLAG-HA-tagged EGFP (codon optimized for efficient gene synthesis) in Flp-In T-REx system. |
| 106340 | pFRT-TO_FH_hDND1_opt | Mammalian expression vector for FLAG-HA-tagged DND1 (codon optimized for efficient gene synthesis) in Flp-In T-REx system. |
| 106341 | pFRT-TO-hNANOS3opt-VH | Mammalian expression vector for NANOS3 C-terminally tagged with V5-HA (codon optimized for efficient gene synthesis) in Flp-In T-REx system. |
| 106342 | pFRT-TO-hNANOS3opt-VH-FH-DND1opt | Mammalian expression vector for NANOS3 C-terminally tagged with V5-HA and FLAG-HA-tagged DND1 (codon optimized for efficient gene synthesis) in Flp-In T-REx system. |
| 106349 | pLVCT-DEST-IPG | Destination vector for gateway cloning. Bicistronic expression of Puro-R fused with EGFP under the control of IRES. Tet elements are inserted in 3'LTR for tTR-KRAB mediated gene suppression. |
| 106350 | pLVE-DEST-IPG | Destination vector for gateway cloning. Bicistronic expression of Puro-R fused with EGFP followed by murine Eef1a1 3'UTR under the control of IRES. loxP sequence is inserted in 3'LTR. |
| 106351 | pLVE-IPG | Bicistronic expression of Puro-R fused with EGFP followed by murine Eef1a1 3'UTR under the control of IRES. loxP sequence is inserted in 3'LTR. |