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pLVCT-DEST-IPG
(Plasmid #106349)

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  • Purpose
    (Empty Backbone) Destination vector for gateway cloning. Bicistronic expression of Puro-R fused with EGFP under the control of IRES. Tet elements are inserted in 3'LTR for tTR-KRAB mediated gene suppression.
  • Depositing Lab
    Thomas Tuschl
  • Depositing Organization
    Rockefeller University
    Located in the United States of America
  • Publication
    Thomas Tuschl Lab Plasmids 2018 (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106349 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLVCT-tTR-KRAB
  • Backbone manufacturer
    Patrick Aebischer & Didier Trono (Addgene plasmid # 11643)
  • Vector type
    Mammalian Expression, Lentiviral, Gateway Cloning
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVCT-DEST-IPG was a gift from Thomas Tuschl (Addgene plasmid # 106349 ; http://n2t.net/addgene:106349 ; RRID:Addgene_106349)
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