YFP-S100A10
(Plasmid #107200)

• Purpose: Expresses YFP-human S100A10 fusion protein for live cell imaging
• Depositing Labs: Volker Gerke, Ursula Rescher
• Publication: Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: S100A10
• Alt name: p11
• Alt name: annexin A2 light chain
• Alt name: Calpactin light chain
• Species: H. sapiens (human)
• Insert Size (bp): 294
• GenBank ID: M_002966.2
• Entrez Gene: S100A10 (a.k.a. 42C, ANX2L, ANX2LG, CAL1L, CLP11, Ca[1], GP11, P11, p10)
• Promoter: CMV
• Tag / Fusion Protein:
  • Yellow Fluorescent Protein (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry