MSGV-1D3-28Z All ITAMs intact
(Plasmid #107226)

• Purpose: Anti-mouse CD19 CAR with CD3 ITAMs intact
• Depositing Labs: James Kochenderfer, Steven Rosenberg
• Publication: Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi: 10.1182/blood-2010-01-265041. Epub 2010 Jul 14.

Backbone

• Vector backbone: MSGV1
• Backbone size w/o insert (bp): 5584
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 1D3-28Z All ITAMs intact
• Alt name: Anti-mouse CD19 CAR with CD3 ITAMs intact
• Species: Synthetic
• Insert Size (bp): 1429
• Mutation: None
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry