pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS
(Plasmid
#107300)
-
PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 107300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCSDest2
- Backbone size w/o insert (bp) 4096
- Total vector size (bp) 7570
-
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTruncated SpCas9 (aa 574-1368) fused to FKBP
-
SpeciesSynthetic; S. pyogenes
-
Insert Size (bp)3474
-
MutationTruncated SpCas9 (aa 574-1368) with R1335K mutation
- Promoter CMV IE94
-
Tags
/ Fusion Proteins
- FKBP (N terminal on insert)
- SV40NLS, 3xHA, c-Myc NLS (C terminal on insert)
- ZFP_VEGFA-TS3 (C terminal on insert)
- 3x Flag, 2x NLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS was a gift from Scot Wolfe (Addgene plasmid # 107300 ; http://n2t.net/addgene:107300 ; RRID:Addgene_107300)