shPBRM1#32-Tet-pLKO-puro
(Plasmid
#107399)
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PurposeLentiviral expression of shRNA targeting PBRM
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneTet-pLKO-puro
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Backbone manufacturerDmitri Wiederschain (Addgene plasmid # 21915)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA targeting PBRM1
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gRNA/shRNA sequenceccggGCACTCAGCTATACCACAAaactcgagttTTGTGGTATAGCTGAGTGCttttt
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SpeciesH. sapiens (human)
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Entrez GenePBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
shPBRM1#32-Tet-pLKO-puro was a gift from William Kaelin (Addgene plasmid # 107399 ; http://n2t.net/addgene:107399 ; RRID:Addgene_107399) -
For your References section:
Inactivation of the PBRM1 tumor suppressor gene amplifies the HIF-response in VHL-/- clear cell renal carcinoma. Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 10.1073/pnas.1619726114. Epub 2017 Jan 12. 10.1073/pnas.1619726114 PubMed 28082722
