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PurposeAn entry vector with Cas9-NLS-P2A-mCherry
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepME
- Backbone size w/o insert (bp) 2726
- Total vector size (bp) 7648
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecas9-NLS-P2A-mCherry
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Insert Size (bp)4922
- Promoter T7
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Tags
/ Fusion Proteins
- SV40 NLS (C terminal on insert)
- P2A-mCherry (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
infusion cloning was used
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-cas9-2A-mCherry was a gift from Qing Deng (Addgene plasmid # 107592 ; http://n2t.net/addgene:107592 ; RRID:Addgene_107592) -
For your References section:
Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish. Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q. Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027. 10.1242/dmm.033027 PubMed 29590639
