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pcDNA5-GFP-PKHD1
(Plasmid #108408)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108408 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKHD1
  • Alt name
    fibrocystin/polyductin
  • Alt name
    FPC
  • Species
    H. sapiens (human)
  • Entrez Gene
    PKHD1 (a.k.a. ARPKD, FCYT, FPC, PKD4, TIGM1)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Depositor Comments

Addgene sequencing results find V1463A, L1870V, S3864P, and Q3899R mutations in PKHD1 compared to the NCBI reference sequence NM_138694.3. The S3864P mutation is specific to this plasmid from the publication. It is unknown how these mutations affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-GFP-PKHD1 was a gift from Gregory Germino (Addgene plasmid # 108408 ; http://n2t.net/addgene:108408 ; RRID:Addgene_108408)
  • For your References section:

    Polyductin undergoes notch-like processing and regulated release from primary cilia. Kaimori JY, Nagasawa Y, Menezes LF, Garcia-Gonzalez MA, Deng J, Imai E, Onuchic LF, Guay-Woodford LM, Germino GG. Hum Mol Genet. 2007 Apr 15. 16(8):942-56. 10.1093/hmg/ddm039 PubMed 17470460