pcDNA5-GFP-PKHD1
(Plasmid #108408)

• Purpose: Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.
• Depositing Lab: Gregory Germino
• Publication: Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56.

Backbone

• Vector backbone: pcDNA5
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKHD1
• Alt name: fibrocystin/polyductin
• Alt name: FPC
• Species: H. sapiens (human)
• Entrez Gene: PKHD1 (a.k.a. ARPKD, FCYT, FPC, PKD4, TIGM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results find V1463A, L1870V, S3864P, and Q3899R mutations in PKHD1 compared to the NCBI reference sequence NM_138694.3. The S3864P mutation is specific to this plasmid from the publication. It is unknown how these mutations affect plasmid function.