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p3187 HPV-16 E2
(Plasmid #10846)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 10846 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HPV-16 E2
  • Alt name
  • Species
    Human papillomavirus
  • GenBank ID
  • Entrez Gene
    E2 (a.k.a. HpV16gp4)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI* (not destroyed)
  • 3′ cloning site Possibly EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HPV-16 E2 (aa1-365) from pJG4-5-derived plasmid. See author's map.

*Note that Addgene's sequence and sequence we have received from requesting labs suggest that the cloning strategy described in the depositor map is not accurate. There is a BamHI site at the 5' of the insert and an EcoRI site at the 3' end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3187 HPV-16 E2 was a gift from Peter Howley (Addgene plasmid # 10846 ; ; RRID:Addgene_10846)
  • For your References section:

    Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins. Yasugi T, Benson JD, Sakai H, Vidal M, Howley PM. J Virol. 1997 Feb . 71(2):891-9. PubMed 8995605