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Addgene

p3037 GST-HPV-6 E6
(Plasmid #10851)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV6b E6
  • Species
    Human papillomavirus
  • Insert Size (bp)
    500
  • Entrez Gene
    E6 (a.k.a. HPV6bgp1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pGEX-5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3037 GST-HPV-6 E6 was a gift from Peter Howley (Addgene plasmid # 10851 ; http://n2t.net/addgene:10851 ; RRID:Addgene_10851)

  • For your References section:

    A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18. Huibregtse JM, Scheffner M, Howley PM. EMBO J. 1991 Dec . 10(13):4129-35. PubMed 1661671
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