pQE30 PTBP1 isoform1
(Plasmid #108591)

• Purpose: Recombinant His tagged protein production
• Depositing Lab: Chris Smith
• Publication: Wollerton et al RNA. 2001 Jun;7(6):819-32.

Backbone

• Vector backbone: pQE30
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3461
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PTBP1 isoform 1
• Alt name: PTB1
• Alt name: hnRNPI
• Species: H. sapiens (human)
• Insert Size (bp): 1602
• Mutation: isoform 1 (isoform c)
• Entrez Gene: PTBP1 (a.k.a. HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
• Tag / Fusion Protein:
  • 6xHis

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asp718 (unknown if destroyed)
• 3′ cloning site: Sal I (unknown if destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: GTTCTGAGGTCATTACTGG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid grows best in XL1 Blue or XL10 Gold. For expression of recombinant protein use E.coli M15 [pREP4] strain.

Addgene NGS result found insert most closely matches PTBP1 isoform c [NP_114368.1].