Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10885||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePP2A Aalpha
SpeciesH. sapiens (human)
Entrez GenePPP2R1A (a.k.a. MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
E64G substitution found in a breast carcinoma.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG-Aalpha E64G was a gift from William Hahn (Addgene plasmid # 10885 ; http://n2t.net/addgene:10885 ; RRID:Addgene_10885)
For your References section:Cancer-associated PP2A Aalpha subunits induce functional haploinsufficiency and tumorigenicity. Chen W, Arroyo JD, Timmons JC, Possemato R, Hahn WC. Cancer Res. 2005 Sep 15. 65(18):8183-92. 10.1158/0008-5472.CAN-05-1103 PubMed 16166293
Map uploaded by the depositor.