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pMIG-Aalpha E64G
(Plasmid #10885)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10885 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIG
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP2A Aalpha
  • Alt name
    PP2A
  • Species
    H. sapiens (human)
  • Mutation
    E64G
  • Entrez Gene
    PPP2R1A (a.k.a. MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

E64G substitution found in a breast carcinoma.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-Aalpha E64G was a gift from William Hahn (Addgene plasmid # 10885 ; http://n2t.net/addgene:10885 ; RRID:Addgene_10885)
  • For your References section:

    Cancer-associated PP2A Aalpha subunits induce functional haploinsufficiency and tumorigenicity. Chen W, Arroyo JD, Timmons JC, Possemato R, Hahn WC. Cancer Res. 2005 Sep 15. 65(18):8183-92. 10.1158/0008-5472.CAN-05-1103 PubMed 16166293