PurposeCre expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108995||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6901
Vector typeCre/Lox ; Cre expression vector
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert nameCre recombinase
Insert Size (bp)1101
- Promoter act15
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDM1488 was a gift from Rob Kay (Addgene plasmid # 108995 ; http://n2t.net/addgene:108995 ; RRID:Addgene_108995)
For your References section:Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM. PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018. 10.1371/journal.pone.0196809 PubMed 29847546