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Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.
Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM
PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108974pDM344shuttle vector
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108975pDM1018shuttle vector, N-terminal mCherry tagging
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108976pDM1019shuttle vector, N-terminal GFP tagging
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108977pDM1020shuttle vector, C-terminal mCherry tagging
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108978pDM1021shuttle vector, C-terminal GFP tagging
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108979pDM1079Knockout cassette Blasticidin
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108980pDM1080Knockout cassette Nourseothricin
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108981pDM1081Knockout cassette Hygromycin
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108982pDM1082Knockout cassette G418
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108983pDM1203extrachromosomal expression vector
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108984pDM1207extrachromosomal expression vector, N-terminal GFP tagging
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108985pDM1208extrachromosomal expression vector, N-terminal mCherry tagging
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108986pDM1209extrachromosomal expression vector, C-terminal GFP tagging
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108987pDM1210extrachromosomal expression vector, C-terminal mCherry tagging
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108988pDM1038Inducible expression vector (dox-on)
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108989pDM1046Inducible expression vector (dox-on), N-terminal mCherry tagging
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108990pDM1047Inducible expression vector (dox-on), N-terminal GFP tagging
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108991pDM1048Inducible expression vector (dox-on), C-terminal mCherry tagging
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108992pDM1049Inducible expression vector (dox-on), C-terminal GFP tagging
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108993pDM1220integrating expression vector
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108994pDM1355generic knockin vector for C-terminal tagging with GFP
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108995pDM1488Cre expression vector
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108996pDM1489Cre expression vector
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108997pDM1501safe haven knock-in plasmid for the act5 locus
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108998pDM1513safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging
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108999pDM1514safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging
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109000pDM1515safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging
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109001pDM1516safe haven knock-in plasmid for the act5 locus, C-terminal mCherry tagging
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113195pPI437extrachromosomal expression vector, N-terminal mScarlet tagging
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113196pPI459extrachromosomal expression vector, C-terminal mScarlet tagging
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113199pPI418shuttle vector, N-terminal mScarlet tagging
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113200pPI457shuttle vector, C-terminal mScarlet tagging
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113203pPI452Inducible expression vector (dox-on) N-terminal mScarlet tagging
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113204pPI460Inducible expression vector (dox-on), C-terminal mScarlet tagging
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113207pPI468integrating expression vector, N-terminal mScarlet tagging
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113208pPI467integrating expression vector, C-terminal mScarlet tagging
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113211pPI419safe haven knock-in plasmid for the act5 locus, N-terminal mScarlet tagging
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113212pPI458safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging
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113215pPI461generic knockin vector for C-terminal tagging with mCherry
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113216pPI464generic knockin vector for C-terminal tagging with mScarlet
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113218pPI191generic knockin vector for C-terminal tagging with GFP
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113219pPI192generic knockin vector for C-terminal tagging with mCherry
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113221pPI184generic knockin vector for C-terminal tagging with mCherry
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113222pPI180generic knockin vector for C-terminal tagging with GFP
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113227pPI278extrachromosomal expression vector, expression of H2B-GFP
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113228pPI226safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry
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113229pPI138extrachromosomal expression vector, expression of LifeAct-GFP
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113230pPI420safe haven knock-in plasmid for the act5 locus, Expression of H2B-mCherry
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113231pPI227safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP
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113232pPI304extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP
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207427pPI143Extrachromosomal expression for mNeonGreen Fusions C-terminal
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207428pPI152Shuttle Vector for mNeonGreen Fusions
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207430pPI159Extrachromosomal expression for mNeonGreen Fusions N-terminal
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207431pPI230Safe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusions
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207432pPI231Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions
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207433pPI450Inducible extrachromosomal overexpression vector - mNeonGreen - N-terminal
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207434pPI462Knock in plasmid for mNeonGreen fusions C-terminal
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207435pPI465REMI integrated Expression for mNeonGreen Fusions N-terminal
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207436pPI470Inducible extrachromosomal overexpression vector - mNeonGreen - C-terminal
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207437pPI471REMI integrated Expression for mNeonGreen Fusions C-terminal
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Antibodies from Article